Key development for liquid biopsy research
A recent publication from the BloodPAC group seeks to standardize pre-analytical variables for liquid biopsies. Titled “Minimum Technical Data Eleme…
Read More Cell-Free DNA Urine Preserve
Cell-Free DNA Urine Preserve is a general purpose reagent in a ready-to-use liquid format that stabilizes all the cell-free DNA in samples for up to 7 days at 6 °C to 37 °C. Once isolated, the cfDNA is suitable for many downstream applications such as qPCR, ddPCR, and other methods used to profile circulating DNA.
Product Description
Cell-Free DNA Urine Preserve is a general purpose reagent in a ready-to-use liquid format that stabilizes cell-free DNA (cfDNA) in urine samples. Samples treated with Cell-Free DNA Urine Preserve are stable up to 7 days at 6 °C to 37 °C. The cfDNA isolated can be used for many downstream applications such as qPCR, ddPCR, and other methods used to profile circulating DNA. The reagent stabilizes all cellular components, including red blood cells and white blood cells present at the time of collection. Cell-Free DNA Urine Preserve enhances the stability of cfDNA and eliminates the lysis of nucleated blood cells and release of cellular genomic DNA which could interfere with accurate cfDNA analysis. Treatment of urine with the liquid preservation reagent also inhibits nuclease mediated nucleic acid degradation during room temperature storage.
Features
- 7-day sample stability when stored at 6 °C to 37 °C
- Contains a PCR compatible blue dye to provide visual reassurance that reagent was added
- Available in single-use plastic ampoules
- Isolated cfDNA is suitable for many downstream applications
Benefits
- Can easily be added to urine samples at time of collection
- Reduces the need for immediate sample extraction
- Room temperature storage reduces costs and complications associated with cold chain shipping
- Inhibits nuclease degradation of cell-free DNA and limits release of genomic DNA, both of which can interfere with analysis
Resources
Instructions (IFU)
Product Information
Certificates
- Cell-Free DNA Urine Preserve CoC Lot 8148 2020-05-28
- Cell-Free DNA Urine Preserve CoC Lot 9119 2021-04-29
- Cell-Free DNA Urine Preserve CoC Lot 9203 2021-07-22
- Cell-Free DNA Urine Preserve CoC Lot 9287 2021-08-30
- Cell-Free DNA Urine Preserve CoC Lot 0006 2021-11-25
- Cell-Free DNA Urine Preserve CoC Lot 0090 2022-03-30
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Stabilization of cfDNA in Urine Using a Preservative Reagent During Sample Processing, Transport, and Storage
Conclusion: The novel urine preservative reagent can stabilize cfDNA in urine and minimize the post-sampling urinary DNA background for an extended period of time at various storage temperatures. This new methodology provides clinical laboratories with great flexibility and convenience in urine sample processing, handing and storage for urinary nucleic acid…
Stabilization of cfDNA and Blood Cells in Urine Using a Preservative Reagent and its Compatibility with Urine Analyzers
Conclusion: The novel urine preservative reagent can stabilize cfDNA and blood cells in urine and minimize the post-sampling urinary DNA background for an extended period of time at various storage temperatures ranging from 6 °C to 37 °C (previous temperature data not shown). Preserved urine samples are generally compatible with urine analyzers….
Ordering Information
| Description | Catalog Number |
|---|---|
| 20 x 5.0 mL | 230216 |
| 5 x 5.0 mL | 230230 |
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Stabilization Products
Streck’s patented portfolio of stabilization solutions enables scientists to mitigate and limit pre-analytical variation in critical samples, ease handling concerns and allow for room temperature storage, cold-chain free shipping and batch processing. Our direct draw blood collection tubes and preservation products for biofluids are recognized worldwide. Target analytes include cell-free DNA, cell-free RNA, clusters of differentiation antigens, extracellular vesicles and cellular nucleic acids.
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