Accurate analysis of cell-free DNA can be compromised by sample handling, shipping and processing, causing lysis of nucleated blood cells and subsequent release of cellular genomic DNA. The preservative reagent contained in Cell-Free DNA BCT reduces the release of cellular genomic DNA. This claim has been substantiated compared to cfDNA isolated from K2EDTA tubes for liquid biopsy next-generation sequencing assays. The blood collection tube is intended for the preservation of cell-free DNA.
Performance characteristics of this device have only been established on the Guardant360 CDx assay.