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Conventional PCR

Step Description Temp (°C) Time (sec)
1 Hot Start The temp must be 0-100 The time must be 0-1000
2 Denature The temp must be 0-100 The time must be 0-1000
3 Anneal The temp must be 0-100 The time must be 0-1000
4 Extension The temp must be 0-100 The time must be 0-1000
5 Final Extension Hold The temp must be 0-100 The time must be 0-1000
Number of Cycles The cycles must be 0-60
Total Time
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Philisa® Rapid PCR

Step Description Temp (°C) Time (sec)
1 Hot Start
2 Denature
3 Anneal
4 Extension
5 Final Extension Hold
Number of Cycles
Total Time

If you would like to further customize your protocol with Philisa, click below to contact our Molecular Specialist.

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